Sustainable Bioproduction of Xanthan Gum by Xanthomonas campestris Using Fructose and Black Soldier Fly Larvae as a Novel Nitrogen Source
DOI:
https://doi.org/10.24191/bioenv.v3i3.95Keywords:
Xanthan gum , Black Soldier Fly Larvae , Sustainable bioproduction , Xanthomonas campestrisAbstract
Xanthan gum is a microbial exopolysaccharide produced commercially through the fermentation of Xanthomonas campestris using carbon sources. It is widely used in industries such as food, medicine, textile, and oil due to its properties as a stabilizer, thickener, and emulsifier. However, the high cost of xanthan production and its environmental impact, particularly when relying on traditional carbon sources like glucose, pose significant challenges. This study explores the use of agricultural waste as a cost-effective carbon source and investigates the potential of Black Soldier Fly Larvae (BSFL) as an alternative nitrogen source for xanthan production. Xanthomonas campestris ATCC 13951 was cultivated in media containing fructose (40 g/L) as the carbon source and varying concentrations of BSFL (2, 4, 6, 8, and 10 g/L) as the nitrogen source. The results demonstrated that the medium containing 2 g/L of BSFL yielded the highest xanthan production at 5.29 g/L, indicating the potential of BSFL as a sustainable nitrogen source for xanthan synthesis. This study highlights the feasibility of using alternative, low-cost substrates to improve the economic and environmental sustainability of xanthan production.
References
Ashour, E. (2000). Optimization of fermentation conditions for xanthan production by Xanthomonas campestris. Journal of Biotechnology, 78(1), 1–10. https://www.researchgate.net/publication/315758872
Bhat, I. M., Wani, S. M., Mir, S. A., & Masoodi, F. A. (2022). Advances in xanthan gum production, modifications and its applications. Biocatalysis and Agricultural Biotechnology, 42, 102328. https://doi.org/10.1016/j.bcab.2022.102328
Cancella, M. J., Cerqueira, A. F. L. W., Teodoro, L. da C., Pereira, J. R., Ludwig, Z. M. da C., Anjos, V. de C., Denadai, Â. M. L., Húngaro, H. M., & Rodarte, M. P. (2024). Xanthan gum produced from milk permeate and deproteinized cheese whey: A comparative analysis with commercial xanthan gums. Biocatalysis and Agricultural Biotechnology, 56, 103053. https://doi.org/10.1016/j.bcab.2024.103053
Kalogiannis, S., Iakovidou, G., Liakopoulou-Kyriakides, M., Kyriakidis, D. A., & Skaracis, G. N. (2003). Optimization of xanthan gum production by Xanthomonas campestris grown in molasses. Process Biochemistry, 39(2), 249–256. https://doi.org/10.1016/S0032-9592(03)00067-0
Lu, S., Taethaisong, N., Meethip, W., Surakhunthod, J., Sinpru, B., Sroichak, T., Archa, P., Thongpea, S., Paengkoum, S., Purba, R. A. P., & Paengkoum, P. (2022). Nutritional composition of black soldier fly larvae (Hermetia illucens L.) and its potential uses as alternative protein sources in animal diets: A review. Insects, 13(9), 831. https://doi.org/10.3390/insects13090831
Nejadmansouri, M., Shad, E., Razmjooei, M., Safdarianghomsheh, R., Delvigne, F., & Khalesi, M. (2020). Production of xanthan gum using immobilized Xanthomonas campestris cells: Effects of support type. Biochemical Engineering Journal, 157, 107554. https://doi.org/10.1016/j.bej.2020.107554
Niknezhad, S. V., Asadollahi, M. A., Zamani, A., & Biria, D. (2016). Production of xanthan gum by free and immobilized cells of Xanthomonas campestris and Xanthomonas pelargonii. International Journal of Biological Macromolecules, 82, 751–756. https://doi.org/10.1016/j.ijbiomac.2015.10.065
Nordin, N.Z, Rashidi, A.R, Dailin, D.J, Abd Malek, R., Izyan Wan Azelee, N., Hasmaliana Abd Manas, N., Selvamani, S., Norulfairuz Abg Zaidel, D., Abd Alsaheb, R. A., Sukmawati, D., El Enshasy, H., & Arab, A. (2020). Xanthan biopolymer in pharmaceutical and cosmeceutical applications: Critical review. Bioscience Research, 17(1), 205–220.
Ozdal, M., & Kurbanoglu, E. B. (2019). Use of chicken feather peptone and sugar beet molasses as low cost substrates for xanthan production by xanthomonas campestris MO-03. Fermentation, 5(1), 8–10. https://doi.org/10.3390/fermentation5010009
Palaniraj, A., & Jayaraman, V. (2011). Production, recovery, and applications of xanthan gum by Xanthomonas campestris. Journal of Food Engineering, 106(1), 1–12. https://doi.org/10.1016/j.jfoodeng.2011.03.035
Quan, J., Wang, Y., Cheng, X., Li, C., & Yuan, Z. (2023). Revealing the effects of fermented food waste on the growth and intestinal microorganisms of black soldier fly (Hermetia illucens) larvae. Waste Management, 171, 580–589. https://doi.org/10.1016/j.wasman.2023.10.002
Rashidi, A.R, Joe Dailin, D., Ramli, S., Zulaiha Hanapi, S., Fatimah Ibrahim, S., & El Enshasy, H. (2023). Variable nitrogen sources effect on Xanthomonas campestris ATCC 13915 ability for xanthan production in culture supplemented with pineapple waste. Bioscience Research, 20(1), 7-12.
Rui, X., Amenorfenyo, D. K., Peng, K., Li, H., Wang, L., Huang, X., Li, C., & Li, F. (2023). Effects of different nitrogen concentrations on co-production of fucoxanthin and fatty acids in Conticribra weissflogii. Marine Drugs, 21(2), 106. https://doi.org/10.3390/md21020106
Wei, M., Li, T., Khan, S., Li, H., Wen, T., Yi, T., & Guo, J. (2024). Effects of black soldier fly larvae on biotransformation and residues of spent mushroom substrate and wet distiller’s grains. Scientific Reports, 14(1), 22392. https://doi.org/10.1038/s41598-024-72959-y
Zakeri, A., Pazouki, M., & Vossoughi, M. (2017). Use of response surface methodology analysis for xanthan biopolymer production by Xanthomonas campestris: Focus on agitation rate, carbon source, and temperature. Iranian Journal of Chemistry and Chemical Engineering, 36(1), 173–183.
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Copyright (c) 2025 Muhammad Safiy Hafiz Sharif, Ahmad Ramli Rashidi, Mohd Zaki Sukor
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