Phytochemical Screening of Selected Ulam: A Preliminary Study
DOI:
https://doi.org/10.24191/bioenv.v4i3.163Keywords:
Phenolic , phytochemical , saponin , ulamAbstract
In Malaysia, ulam refers to plant species commonly consumed raw as fresh vegetables, particularly within the Malay community. Despite their widespread consumption, information on the phytochemical constituents of many ulam species remains limited. This study qualitatively screened seven selected species: Cosmos caudatus, Psidium guajava, Alternanthera sissoo, Leucaena leucocephala, Morinda citrifolia, Garcinia atroviridis, and Oenanthe javanica. Fresh samples were cleaned, oven-dried, ground into powder, and extracted with methanol. The extracts were screened for alkaloids, flavonoids, glycosides, phenolic compounds, saponins, tannins, and sterols. Phenolic compounds were detected in all seven species, whereas saponins were not detected under the assay conditions used. Alternanthera sissoo and L. leucocephala tested positive for all investigated phytochemical groups except saponins. These findings provide preliminary comparative data on the phytochemical profiles of the selected ulam species. Further chemical characterisation and biological assays, including antimicrobial and antifungal testing, are needed to evaluate their potential biological activities.
References
Abdulkader, O. M., Sharaf, A. E. A., Fouda, H. M. & Elhaw, M. H. (2022), Phytoconstitutes investigation of Chenopodium ambrosioides Linn. and gas chromatography with mass spectroscopy material analysis. Materials Today: Proceedings, 61, 992–997. https://doi.org/10.1016/j.matpr.2021.10.280
Alfanaar, R., Manurung, T. W. & Ariefin, M. (2024), Modelling of flavanoid derivatives from Leucaena leucocephala as anti-UV. Stannum: Jurnal Sains dan Terapan Kimia, 6(1), 21–27.
Asiandu, A. P., Sari, W., Sari, S. W. & Majid, A. S. A. (2023), The anticancer properties of guava leaves (Psidium guajava L.) and turmeric rhizome (Curcuma longa L.) against breast cancer: A literature study. Biology, Medicine, & Natural Product Chemistry, 12(1), 407–412. https://doi.org/10.14421/biomedich.2023.121.407-412
Du, Y., Fu, X., Chu, Y., Wu, P., Liu, Y., Ma, L., Tian, H. & Zhu, B. (2022), Biosynthesis and the roles of plant sterols in development and stress responses. International Journal of Molecular Sciences, 23(4), 2332. https://doi.org/10.3390/ijms23042332
Haralampidis, K., Trojanowska, M. & Osbourn, A. E. (2002), Biosynthesis of triterpenoid saponins in plants. In: Dutta, N.N. et al. (Eds) History and Trends in Bioprocessing and Biotransformation, Springer Berlin, Heidelberg, pp. 31–49. https://doi.org/10.1007/3-540-44604-4_2
Hatta, F. A. M., Ali, Q. A. M., Othman, R., Kashim, M. I. A. M., Ramya, R., Wan Sulaiman, W. S. H. & Latiff, N. H. M. (2024), Unlocking the antioxidant potential of Malaysian traditional salad (ulam) as a Halal natural carotenoid source. Malaysian Journal of Chemistry, 26(6), 217–227. http://irep.iium.edu.my/id/eprint/117143
Hunt, T., Pontifex, M. G. & Vauzour, D. (2024), (Poly)phenols and brain health—beyond their antioxidant capacity. FEBS Letters, 598(24), 2949–2962. https://doi.org/10.1002/1873-3468.14988
Jamal, J. A. & Husain, K. (2022), Traditional Malay ulam for healthy ageing. In: Goh, C.S., Bodeker, G. & Kariippanon, K. (Eds) Healthy Ageing in Asia, CRC Press, Boca Raton, USA. pp. 135–149. https://doi.org/10.1201/9781003043270
Jayakumari, S., Vijayalakshmi, A., Anandhi, N., Mounisha, B., Sameer, M. M. & Yogeshwaran, V. (2023), Wound healing and cytotoxic effects of tannin rich fraction of Psidium guajava L. leaves. Annals of Phytomedicine, 12(2), 707-712. https://doi.org/10.54085/ap.2023.12.2.83
Kumar, A., P, N., Kumar, M., Jose, A., Tomer, V., Oz, E. & Oz, F. (2023), Major phytochemicals: Recent advances in health benefits and extraction method. Molecules, 28(2), 887. https://doi.org/10.3390/molecules28020887
Lumingkewas, A. M. & Unitly, A. J. A. (2023), Identification of phytochemical compounds antioxidant leaf extract and clove flower extract (Syzygium aromaticum). Jurnal Penelitian Pendidikan IPA, 9(10), 8533–8538. https://doi.org/10.29303/jppipa.v9i10.4927
Mazumder, M. A. R., Tolaema, A., Chaikhemarat, P. & Rawdkuen, S. (2023), Antioxidant and anti-cytotoxicity effect of phenolic extracts from Psidium guajava Linn. leaves by novel assisted extraction techniques. Foods, 12(12), 2336. https://doi.org/10.3390/foods12122336
Ranaweera, C. B., Maheshwaran, L., Nadarajah, L., Senadeera, S. P. N. N., Chandana, A. K. & Pathirana, R. N. (2024), Phytochemical testing methodologies and principles for preliminary screening/qualitative testing. Asian Plant Research Journal, 12(5), 11–38. https://doi.org/10.9734/aprj/2024/v12i5267
Samudra, S. R., Fitriadi, R. & Marhaeni, B. (2021), Antifouling-bacterial potentials of kenikir (Cosmos caudatus) and bandotan (Ageratum conyzoides) leaf extracts in freshwater environment. Journal of Aquaculture and Fish Health, 10(2), 213–220. https://doi.org/10.20473/jafh.v10i2.22652
Senjaya, A. A., Supariani, N. N. D. & Sirat, N. M. (2024), Phytochemicals and toxicity of the extract from Cosmos caudatus leaves. Biomedical and Pharmacology Journal, 17(3), 2055–2064. https://doi.org/10.13005/bpj/3007
Sivapragasam, G., Ragavan, N. D., Suryadevara, N., Sridewi, N., Al Obaid, S., Alharbi, S. A. & Arulselvan, P. (2024), A comparative phytochemical characterization of Moringa oleifera plant parts by different solvent extraction. Indian Journal of Pharmaceutical Education & Research, 58, s648-s659. https://doi.org/10.5530/ijper.58.2s.69
Sun, W. & Shahrajabian, M. H. (2023), Therapeutic potential of phenolic compounds in medicinal plants—natural health products for human health. Molecules, 28(4), 1845. https://doi.org/10.3390/molecules28041845
Ushie, O. A., Kendeson, A. C., Longbab, B. D., Ogofotha, P. U., Nongu, S. T. & Bako, B. (2022), Profiling phytochemicals and antimicrobial activities of hexane and acetone crude extracts of Sterculia setigera leaf. Journal of Chemical Society of Nigeria, 47(6), 1296-1302. https://doi.org/10.46602/jcsn.v47i6.827
Downloads
Published
How to Cite
Issue
Section
Categories
License
Copyright (c) 2026 Liliwirianis Nawi

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.


